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Cell Biology
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Membrane Potential Assay Kits
JC-1, Mitochondrial membrane potential dye
JC-1, Mitochondrial membrane potential dye from Abcam
Be the first to write a review!
Citations:
(11)
Supplier Page
Supplier Page from
Abcam for
JC-1, Mitochondrial membrane potential dye
Description
Mitochondrial membrane-potential dye
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
JC-1, Mitochondrial membrane potential dye
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab141387
Quantity
5 mg
Purity
0.98
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Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (11)
(1)
HADHA alleviates hepatic steatosis and oxidative stress in NAFLD via inactivation of the MKK3/MAPK pathway
Molecular Biology Reports
February 1, 2023
Jiexia Ding, Lili Wu, Guoxian Zhu, Jing Zhu, Pingping Luo, Youming Li
and liver tissues Mitochondrial depolarization was analysed with an MMP
assay
kit with JC-1 (
Abcam
,
ab141387
) according to the manufacturer’s instructions. Mitochondrial JC-1 monomers (green) and aggregates (red)
(2)
SPY1 inhibits neuronal ferroptosis in amyotrophic lateral sclerosis by reducing lipid peroxidation through regulation of GCH1 and TFR1
Cell death and differentiation
February 1, 2023
Wang D, Liang W, Huo D, Wang H, Wang Y, Cong C, Zhang C, Yan S, Gao M, Su X,
et al
.
Mitochondrial membrane
potential (MMP) was detected through
incubation
with the JC-1
fluorescence
probe (5 μM,
Abcam
,
ab141387
) but followed by the analysis using a
flow cytometer
(FC500, Beckmancoulter, USA). JC-1 aggregates showed
(3)
Oocytes maintain ROS-free mitochondrial metabolism by suppressing complex I
Nature
July 1, 2022
Rodríguez-Nuevo A, Torres-Sanchez A, Duran JM, De Guirior C, Martínez-Zamora MA, Böke E.
tetramethylrhodamine ethyl ester perchlorate (TMRE
) (
Thermo
,
T669
), or 45 min in 4 µM JC-1 (
Abcam
,
ab141387
). Cells were then washed and imaged in 35-mm glass-bottom MatTek dishes. Oxygen consumption rate Oxygen
(4)
Inhibition of miR-421 Preserves Mitochondrial Function and Protects against Parkinson’s Disease Pathogenesis via Pink1/Parkin-Dependent Mitophagy
Disease Markers
May 10, 2022
Xiaolin Dong, Xianghua He, Li Yang, Qingyun Li, Yanming Xu
Measurement The Δψm was analyzed by measuring the
fluorescence
intensity of cells stained with JC-1 dye (
Abcam
,
ab141387
). SH-SY5Y cells were trypsinized, washed with PBS buffer, and resuspended in DMEM. SH-SY5Y cells were
(5)
Ginsenoside Rg3 alleviates septic liver injury by regulating the lncRNA TUG1/miR-200c-3p/SIRT1 axis
Journal of inflammation (London, England)
December 20, 2021
Wu P, Yu X, Peng Y, Wang QL, Deng LT, Xing W.
(Ex/Em = 510/580 nm). Detection of mitochondrial transmembrane potential (MTP) JC-1 (CBIC2) dye (
ab141387
,
Abcam
) was employed to
measure
the MTP of
human
primary
hepatocytes
. JC-1 (10 μM) in DMEM was added
(6)
Inhibition of Autophagy Prevents Panax Notoginseng Saponins (PNS) Protection on Cardiac Myocytes Against Endoplasmic Reticulum (ER) Stress-Induced Mitochondrial Injury, Ca
2+
Homeostasis and Associated Apoptosis
Frontiers in pharmacology
January 1, 2021
Chen J, Li L, Bai X, Xiao L, Shangguan J, Zhang W, Zhang X, Wang S, Liu G.
assessed using 5, 5′, 6, 6′-tetrachloro-1, 1′, 3, 3′-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) (
ab141387
,
Abcam
, United States). In details,
cardiomyocytes
were seeded in a 96-well black frame plate at the
(7)
Mir-141-3p Regulates Apoptosis and Mitochondrial Membrane Potential via Targeting Sirtuin1 in a 1-Methyl-4-Phenylpyridinium in vitro Model of Parkinson’s Disease
BioMed Research International
November 6, 2020
Yumin Zheng, Li Dong, Na Liu, Xiaoguang Luo, Zhiyi He
Potential. Δψm was analyzed via measuring the fluorescent intensity of cells stained with JC-1 dye (
ab141387
,
Abcam
, Cambridge, UK). The
PC12 cells
were treated with trypsin, washed with PBS buffer, and resuspended
(8)
Structure-function relationships of HDL in diabetes and coronary heart disease
JCI insight
January 16, 2020
Cardner M, Yalcinkaya M, Goetze S, Luca E, Balaz M, Hunjadi M, Hartung J, Shemet A, Kränkel N, Radosavljevic S,
et al
.
of HDL for an additional 2 hours. MMP was monitored using the JC-1 fluorescent dye (
Abcam
, catalog
ab141387
) that accumulates in
mitochondria
in a voltage-dependent manner (dye was loaded for 30 minutes prior
(9)
Sulfated polysaccharide of Sepiella maindroni ink targets Akt and overcomes resistance to the FGFR inhibitor AZD4547 in bladder cancer
Aging
September 23, 2019
Shan L, Liu W, Zhan Y.
and cells were then harvested and stained with the
mitochondrial membrane
potential reporter JC-1 (
ab141387
,
Abcam
, Cambridge, UK). JC-1
fluorescence
was assessed by
flow cytometry
in three individual experiments. Xenograft
(10)
Active Vitamin D and Vitamin D Receptor Help Prevent High Glucose Induced Oxidative Stress of Renal Tubular Cells via AKT/UCP2 Signaling Pathway
BioMed research international
January 1, 2019
Zhu X, Wu S, Guo H.
to wash the cells. The cells were incubated with
ROS
(
MA5-26760
,
Life technologies
), JC-1 antibody (
ab141387
,
Abcam
) and Annexin V/PI(
556547
,
BD
)at room temperature for 30 min,
and flow cytometry
analysis was
(11)
Soluble guanylate cyclase activation during ischemic injury in mice protects against postischemic inflammation at the mitochondrial level
American Journal of Physiology-Gastrointestinal and Liver Physiology
May 1, 2016
Derek Z. Wang, Allan W. Jones, Walter Z. Wang, Meifang Wang, Ronald J. Korthuis
HEPES-HCO3-buffered salt solution, as in our previous study (29). The suspension was incubated with 4 g/ml JC-1 (
ab141387
;
Abcam
) for 15 min in the dark at room temperature. The
fluorescence
was measured in duplicate at excitation/emission
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JC-1, Mitochondrial membrane potential dye from Abcam
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